Each steponeplus realtime pcr system is factorycalibrated for optical and thermal accuracy, so simply remarkable realtime pcr results can be obtained right out of the box. The plexor hy systemaf is a novel realtime pcr system for quantification of total human autosomal and human male y dna within a sample. Help from within applied biosystems 75007500 fast realtime pcr software v2. Researchers use applied biosystems integrated systems for sequencing, flow cytometry, and real. Applied biosystems realtime pcr rapid assay development guidelines are a series of design and experimental guidelines aimed towards maximizing success while reducing upfront time and costs of. The 7500 real time pcr system is a powerful platform for labs requiring superior performance and maximum dye versatility. The stepone and steponeplus systems software contains. This remarkably simple realtime pcr system is designed with an lcd touchscreen and a userfriendly interface that brings the power of genetic studies to researchers new to realtime pcr fig 1. Applied biosystems 75007500 fast realtime pcr system getting started guide for relative standard curve and comparative c t experiments 4387783 applied biosystems 7900ht fast realtime pcr system relative quantitation using comparative c t getting started guide 4364016 applied biosystems stepone and steponeplus realtime pcr.
The applied biosystems analysis software can import and analyze data generated by any of the supported instruments listed in the following table. Applied biosystems 7500 realtime pcr system thermo fisher. Intended for laboratory staff and principal investigators who perform experiments using the stepone or steponeplus system. The hrm software is available on the 7500 fast realtime pcr system, which delivers precise results in a standard 96well format. Applied biosystems 7500 fast realtime pcr system thermo. Plexor hy system for the applied biosystems 7500 and 7500. The applied biosystems stepone and steponeplus realtime pcr systems offer intuitive software, fast and standard runs, and are configured for pcfree or networked operation. Field application specialist doug rains offers advice for fixing common software setup mistakes when performing realtime pcr. Applied biosystems viia 7 realtime pcr system thermo.
The overall size and footprint of the abi 7500 was made more compact. Steponeplus realtime pcr systems getting started guide for genotyping experiments explains how to perform experiments on the stepone and steponeplus systems. Perform standard pcr in less than 2 hours and fast pcr reactions in less than 40 minutes intuitive, flexible software. This applied biosystem prism 7000 sequence detection system is in excellent condition.
Check out the most popular applied biosystems products for genetic analysis. This instrument is capable of quantitative detection using realtime analysis. Specifically designed with a user friendly, yet powerful interface, they are ideal for both new and experienced realtime pcr researchers. The applied biosystems analysis software is a secure web application for analysis of data that are generated on thermo fisher scientific realtime pcr instruments. Applied biosystems quantstudio 12k flex realtime pcr system applied biosystems quantstudio 1 realtime. Field application specialist doug rains offers advice for fixing common software setup. This real time pcr system combines pre boxed or custom assays, and advanced thermal cycling systems with responsive software for a vast array of applications. This system is a sophisticated platform for users who require extended capabilities and maximum versatility. Applied biosystems 7500 fast, 7500 and 7300 realtime pcr systems. G161544 applied biosystems 4376592 steponeplus realtime.
Interact with the latest advancements in touch screen usability, access your. The applied biosystems 7500 fast real time pcr offers an intuative and flexible solution for rapid detection and quantification of nucleic acids. Applied biosystems relative quantitation analysis module. The applied biosystems 7500 realtime pcr system is a 3rd generation of thermocycling technology that allows quanitation of product generation in real time. The 7500 fast dx realtime pcr instrument delivers the performance required for highquality results in a 96well format. Applied biosystems stepone steponeplus realtime pcr systems. Applied biosystems steponeplus realtime pcr system, laptop. Applied biosystems stepone realtime pcr system fisher. Applied biosystems 7900ht fast realtime pcr system thermo. The abi prism 7000 sequence detection system qpcr rtpcr 7000 sds instrument is a secondgeneration sequence detection instrument capable of quantitative and qualitative detection with fluorescentbased pcr chemistries.
Applied biosystems stepone realtime pcr software stepone software. Feb 24, 2015 the abi 7500 is a real time pcr that delivers accurate results in less than 30 minutes. Prior to downloading any software to a computer used for instrument controloperation, please. This system is a sophisticated platform for users who.
The abi 7500 is a real time pcr that delivers accurate results in less than 30 minutes. Applied biosystems stepone and steponeplus realtime pcr systems remarkably simple systems. In no event shall applied biosystems be liable for incidental, special, multiple, or consequential damages in connection with or arising from the use of this document. G161544 applied biosystems 4376592 steponeplus realtime pcr. Since we pioneered realtime pcr over a decade ago, applied biosystems has continued to develop the technology to provide more powerful solutions to labs of all sizes.
The software provides projectbased analysis of realtime and endpoint data for various quantitative and qualitative pcr applicatio. Utilizing robust led based 3color optical recording, the stepone realtime pcr system is designed to deliver precise, quantitative realtime pcr results for a variety of genomic research applications. The steponeplus realtime pcr system can be used via a pc, with a pc connected to a local area network lan, or as a standalone instrument pcfree. Applied biosystems 7500 realtime pcr system, laptop.
The applied biosystems 7300 real time pcr system is an economical solution setting the standard for labs requiring a quality platform at an affordable price. The software versions listed in the table represent only those tested for use with the applied biosystems software. Applied biosystems steponetm and steponeplustm realtime pcr systems site preparation guide 7 preface obtaining information from the help system the stepone software has a help system that. The hrm software is available on the 7500 fast realtime pcr system, which delivers precise results in a standard 96well. These 96 well realtime pcr systems offers four color optical recording allowing for faster measurement. The abi 7500 is the predecessor of the abi 5700, made as the lower throughput version of the abi 7900ht. Which version of the applied biosystems 7500 realtime pcr system software can be used to run the investigator. Researchers use applied biosystems integrated systems for sequencing, flow cytometry, and realtime, digital and end point pcrfrom sample prep to data analysis. Applied biosystems realtime pcr instruments thermo. Laboratory equipment measuring instruments bicycle accessories software control unit. Features of the the stepone realtime pcr system include.
Applied biosystems steponetm and steponeplustm realtime pcr systems site preparation guide 7 preface obtaining information from the help system the stepone software has a help system that d escribes how to use each feature of. Primerdesign ltd covid19 genesig realtime pcr assay. Prior to downloading any software to a computer used for instrument controloperation, please consult with your service and support. The applied biosystems 7500 realtime pcr system is a 96well, fivecolor platform that uses fluorescencebased polymerase chain reaction pcr reagents to analyze items. Every consumable run on a thermo fisher scientific realtime qpcr instrument requires the creation of one or more experiment files that store the associated data. Designed for compatibility with applied biosystems taqman assays and the flexibility of unlimited realtime chemistry choices, we make it easy for you to get started with your experiments.
A tutorial, using example experiment data provided with the applied biosystems stepone realtime pcr software stepone software. Relative quantitation rq algorithms in applied biosystems realtime pcr systems software. Top sites applied biosystems real time pcr 2019 latest. Applied biosystems abi 7500 real time pcr video youtube. The 7500 realtime pcr system is a powerful platform for labs requiring superior performance and maximum dye versatility. The 7300 is a very reliable platform with a high quality peltierbased heating block providing uniform heating and cooling of all the wells.
Applied biosystems pioneered the development of realtime pcr in 1995, enabling highthroughput, quantitative gene expression. Applied biosystems abi 7500 fast realtime pcr system. With our applied biosystems realtime pcr platforms, you get true value with excellent performance, reliability, and worldclass support. Applied biosystems stepone and steponeplus realtime pcr systems getting started guide for 23 presenceabsence experiments notes. Prior to downloading any software to a computer used for instrument controloperation, please consult with your service and support representative. May 10, 2005 the applied biosystems 7500 realtime pcr system is a 3rd generation of thermocycling technology that allows quanitation of product generation in real time. Applied biosystems 7500 fast, 7500 and 7300 realtime pcr.
User bulletin applied biosystems realtime pcr systems july 2007 subject. Applied biosystems 7500 realtime pcr system, laptop fisher. Applied biosystems 7500 fast realtime pcr system, laptop. Applied biosystems realtime pcr rapid assay development. Applied biosystems steponeplus realtime pcr system. The applied biosystems 7500 is todays top choice in rt pcr. Disclaimer the text above is not a recommendation to uninstall realtime pcr software v2. Notes applied biosystems 75007500 fast realtime pcr system getting started guide for standard curve experiments. Fixing software setup mistakes in realtime pcr setponeplus asktaqman ep 10. Applied biosystems 7500 realtime pcr system thermo.
Applied biosystems 7900ht fast realtime pcr system and. Real time pcr model applied biosystems 7500 now on sale at the lab world group. The information on this page is only about version 2. The applied biosystems analysis software can import and analyze experiment files eds and sds that are generated by various thermo fisher scientific realtime qpcr instruments. Watch the video, find your best fit, or request a quote. The applied biosystems stepone and steponeplus realtime pcr systems offer intuitive software, fast, and are configured for pcfree or networked operation. Realtime pcr system supported software versions file extension applied biosystems 7900 ht fast realtime pcr system v2. Applied biosystems shown were made automatically by the desktop software. The applied biosystems realtime pcr software runs off of windows xp platform and even works with windows 7. The calibrate function is also available in the control console. Applied biosystems 7500 getting started pdf download. The applied biosystems high resolution melting hrm software is the easiest to use melting analysis software enabling realtime pcr melt curve assays to be used more accurately for mutation scanning and genotyping. Applied biosystems 7500 realtime pcr system biocompare.
Realtime pcr videos scientific videos thermo fisher. The applied biosystems steponeplus realtime pcr system, makes it simple and easy to get highquality realtime pcr results. Applied biosystems stepone steponeplus realtime pcr. Advanced software and instrumentation for performing a wide array of genomic assays. Applied biosystems 7900ht fast realtime pcr system and sds. Applied biosystems instruments, assays, and reagents have been trusted in the lab for over 20 years. Applied biosystems 75007500 fast realtime pcr system getting.
View online or download applied biosystems 7500 installation and maintenance manual, getting started, getting started manual. Feb 22, 2016 real time pcr model applied biosystems 7500 now on sale at the lab world group. Data sets can be quickly shared and large files can be sent securely around campus or around the world. Prior to downloading any software to a computer used for instrument controloperation, please consult.
These factorycalibrated onestep systems make it easy to step up to highperformance qpcr. This technical manual describes the plexor hy system and provides instructions for use with the applied biosystems 7500 and 7500 fast realtime pcr systems, including reaction setup, realtime. The applied biosystems steponeplus is a realtime pcr system designed to meet your needs. The software provides projectbased analysis of realtime and endpoint data for various quantitative and qualitative pcr applications. Applied biosystems, a leader in technology solutions for life science research, introduces its latest innovations in realtime pcrthe stepone 48well and steponeplus. Proven performance and superior data quality at an excellent value the 7300 real time pcr system balances exceptional quality with budget sensitivity.
Perform standard pcr in less than 2 hours and fast pcr reactions in less than 40 minutes intuitive, flexible software and wizards guide new users through. May 11, 2009 to achieve high quality results of qpcr, a superb apparatus is needed and this is where you can turn to the applied biosystems 7300 real time pcr system. These factorycalibrated systems make it easy to step up to highperformance qpcr. An example of data from the desktop software is shown by a maize genotyping experiment. Applied biosystems 7500 realtime pcr system software version 2. Application note quantstudio 3 and 5 realtime pcr systems. The steponeplus can even assist you with selecting and ordering pcr reagents needed to run your experiment.
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